Conjugation

Part:BBa_K1510925:Design

Designed by: Kao Jung Chang   Group: iGEM14_NYMU-Taipei   (2014-10-09)


Fragrance generator3: branched-chain amino acid transaminase (BAT2) and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1048
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.



Source

branched-chain amino acid transaminase derived from the BAT2 gene from Saccharomyces cerevisiae and BBa_B0034 is from 2014 igem distribution.

References